In vitro antioxidant and cytotoxicity studies of Curcuma.
The MTT assay is a colorimetric assay for measuring cell metabolic activity. It is based on the ability of nicotinamide adenine dinucleotide phosphate (NADPH)-dependent cellular oxidoreductase enzymes to reduce the tetrazolium dye MTT to its insoluble formazan, which has a purple color (Fig. 10.1 ). This assay therefore measures cell viability.In more fundamental research where the effects of perturbation factors are tested on cellular phenotype, gene, or protein expression, the cytotoxic effects of the perturbing factor must first be investigated via a cell viability assay. The viability assay most commonly used throughout the world is the MTT assay, first described by Tim Mosmann.A collection of MTT Assay Protocols for research, provided by Invitrogen.
MTT Proliferation Assay Protocol ! 1 June 15 Background - Traditionally, the determination of cell growth is done by counting viable cells after staining with a vital dye. Several approaches have been used in the past. Trypan blue staining is a simple way to evaluate cell membrane.The MTT assay was less reliable than CVS. The MTT test of Selol and 2-oxoheptyl ITC, which affected the ROS level and MTT reduction rate, gave false negative (2-oxoheptyl ITC) or false positive (Selol) results. As a consequence, the MTT assay identified an antagonistic interaction between Selol and ITC, while the metabolism-independent CVS test.
It is a quantitative assay that allows rapid and convenient handling of a high number of samples. The Cell Proliferation Kit I (MTT) can be used for multiple applications, such as, Quantification of cell growth and viability. 1,3,5-7; Measurement of cell proliferation in response to growth factors, cytokines and nutrients. 1-3,6,8-12 (see fig. 3).
